gcamp6f mice  (Jackson Laboratory)

Journal: bioRxiv

Article Title: Cell-autonomous mitochondrial calcium flux governs oligodendrocyte regeneration

doi: 10.1101/2025.11.18.689087

Figure Lengend Snippet: ( a ) Experimental timeline. Mice expressing GCaMP6f in oligodendroglia were implanted with a gradient index (GRIN) lens above the corpus callosum of the M1 motor cortex, ∼1.8 mm below the brain surface, and exposed to the CPZ diet for 6 weeks. UCLA Miniscope V4 baseplating was performed in the 5th week of CPZ demyelination and microendoscopy imaging was performed in weeks 5 and 6 of demyelination (orange) as well as during weeks 1 to 6 of remyelination (gray). ( b ) Miniscope recordings were performed in an open field and locomotion behavior was monitored. ( c,d ) Representative projection images of output by Occam with designated active ROIs (white lines) detected in several substacks as obtained with the Miniscope configuration of Occam (File S1) . Two substack projections obtain from image stacks are shown per week ( c ). Ca 2+ activity traces ( c , bottom) and quantification ( d ) of in vivo oligodendroglial Ca 2+ imaging during de-and re-myelination (not significant; one-way repeated measures ANOVA). ( e ) Open field locomotion maps during in vivo Ca 2+ imaging, recorded under demyelination and remyelination conditions in the same mouse. ( f ) Correlations between Ca 2+ signals and distance traveled, speed and motor activity duration (not significant Pearson correlations) during remyelination. Data represented as mean ± s.e.m. Scale bars 100 µm ( b ) and 20 µm ( c ).

Article Snippet: Adult Pdgfrα CreERT( ± ); Gcamp5-tdTomato Lox/Lox and Pdgfrα CreERT( ± ) ;Gcamp6f Lox/Lox mice were intraperitoneally injected with tamoxifen (MP Biomedicals Germany GmbH, CAS: 10540-29-1) to induce the expression of GCaMP5 or GCaMP6f in OPCs and their progeny, respectively.

Techniques: Expressing, Imaging, Activity Assay, In Vivo

Journal: bioRxiv

Article Title: Cell-autonomous mitochondrial calcium flux governs oligodendrocyte regeneration

doi: 10.1101/2025.11.18.689087

Figure Lengend Snippet: ( a ) Experimental timeline. Callosal LPC-induced lesions were induced in control mice (Pdgfrɑ CreER(-/-) ;hM3D(Gq)-YFP lox/+ ;GCaMP6f lox/lox ) and experimental mice (Pdgfrɑ CreER(+/-) ;hM3D(Gq)-YFP lox/+ ;GCaMP6f lox/lox mice) which express both GCaMP6f and the hM3D(Gq) receptor in oligodendroglial cells (Inset: OLIG2 + YFP + cells). Mice were either used to prepare acute slices at 7 dpi or received i.p. CNO injections (1 mg/kg) at 7, 9, 11 and 13 dpi before perfusion at 14 dpi. ( b ) Representative projection image output by Occam with designated active ROIs (white lines) and Ca 2+ traces (middle) from a lesion at 7 dpi. CNO bath application significantly increased Ca 2+ activity compared to baseline. Dot plots (right) showing significant Ca 2+ activity increases in the presence of CNO in lesions (p-value from Wilcoxon matched-pairs signed rank test). ( c ) Representative image of immunofluorescence staining for MBP (magenta), OLIG2 (green) and CC1 (white) in a callosal lesion (dashed line). Insets from white rectangle shows an OLIG2 + CC1 - OPC (open arrow) and mature OLIG2 + CC1 + OL (closed arrow). ( d-f ) Densities of OLIG2 + lineage cells ( d ), OLIG2 + CC1 - OPCs ( e ) and OLIG2 + CC1 + OLs ( f ) in Cre -/- (control) and Cre +/- mice following CNO injections. ( g ) Percentages of OLIG2 + cells that are OPCs (OLIG2 + CC1 - ) or mature OLs (OLIG2 + CC1 + ) did not differ between CNO-treated mice with and without oligodendroglial hM3D(Gq) receptor expression. p-values from mixed linear models with type II likelihood ratio tests ( d-g ). Data is presented as mean ± s.e.m. with open circles representing slices and closed circles averages per mouse ( d,f ). Not significant n.s. Scale bars are 5 µm in ( a ), 10 µm in ( b ) and 50 µm and 10 µm in ( c ).

Article Snippet: Adult Pdgfrα CreERT( ± ); Gcamp5-tdTomato Lox/Lox and Pdgfrα CreERT( ± ) ;Gcamp6f Lox/Lox mice were intraperitoneally injected with tamoxifen (MP Biomedicals Germany GmbH, CAS: 10540-29-1) to induce the expression of GCaMP5 or GCaMP6f in OPCs and their progeny, respectively.

Techniques: Control, Activity Assay, Immunofluorescence, Staining, Expressing

Journal: Frontiers in Pharmacology

Article Title: Kininogen enhances seizure susceptibility in mice possibly through bradykinin-induced modulation of calcium transients in glutamatergic and GABAergic neurons

doi: 10.3389/fphar.2025.1509837

Figure Lengend Snippet: Bradykinin alters PTZ-induced calcium spikes in transgenic mice. (A) The timeline ( left ) and the setup ( right ) of the calcium imaging experiment; Scale bar, 0.5 mm for cranial window image. (B) ( Upper ) Representative images of GCaMP6f-tagged L2/3 cortical excitatory neurons from one animal from each group (average of 1205 frames from the 11–15 min timeline after PTZ treatment), Scale bar: 25 μm; ( middle ) Representative traces from two different time points from two different L2/3 cortical excitatory neurons; ( bottom ) and the % changes in the mean number of calcium events from L2/3 cortical excitatory neurons of the motor cortex after PTZ treatment (BDK vs. PBS). (C) ( Upper ) Representative images of GCaMP6f-tagged L2/3 cortical parvalbumin + inhibitory interneurons from one animal from each group (average of 1205 frames from the 11–15 min timeline after PTZ treatment), Scale bar: 25 μm; ( middle ) Representative traces from two different time points from two different L2/3 cortical parvalbumin + inhibitory interneurons; ( bottom ) and the % changes in the mean number of calcium events from L2/3 cortical parvalbumin + inhibitory interneurons of the motor cortex after PTZ treatment (BDK vs. PBS). Abbreviations: BDK, bradykinin; PBS, phosphate-buffered saline; PTZ, pentylenetetrazole.

Article Snippet: Thy1-GCaMP6f mice were the result of backcrossing Thy1-GCaMP6f males (JAX # 024276) with WT females, while PV Cre -GCaMP6f mice were the result of backcrossing the PV Cre (JAX # 008069) males with Ai95 (RCL-GCaMP6f)-D (JAX # 024105) females.

Techniques: Transgenic Assay, Imaging, Saline

Journal: Scientific Reports

Article Title: ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions

doi: 10.1038/s41598-025-00953-z

Figure Lengend Snippet: Expression of Ano1 transcript in urethral tissues and ANO1 protein in mouse urethral interstitial cells. ( A ) Real-time PCR analyses showing the gene expression of Ano1 normalized to β-actin gene expression (endogenous control) in male, female urethra and proximal colon. Fold change was calculated by taking proximal colon as a calibration control (2 − ΔΔ CT ). Data represents the comparison of Ano1 fold change expression relative to β-actin in male ( n = 3), female urethra ( n = 3) and proximal colon ( n = 3). ( B ) Representative images of double immuno-labelling for anti-GFP (ICC-LC, green) and ANO1 (red) in Kit-GCaMP6f mouse urethra ( n = 5) captured at 40x magnification. ( C ) Representative images of PDGFRα + cells (green) and ANO1 (red) in WT male mouse urethra ( n = 3) captured at 20x magnification. Scale bar: 50 μm in all panels.

Article Snippet: GCaMP6f lox/+ mice (B6; 129 S-Gt(ROSA) 26Sortm95.1(CAG−GCaMP6f)Hze /J) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and were crossed with Kit-iCre expressing mice (Kit-tm1cre/ER T2 Dsa, gifted from Dr. Dieter Saur of the Technical University Munich, Germany) to generate Kit-GCaMP6f mice for conditional expression of GCaMP6f in c-Kit + cells as described previously .

Techniques: Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Control, Comparison